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TFIIF is a [[Eukaryotic|eukaryotic]] [[Transcription factor|transcription factor]] often found associated with [[RNA polymerase II|RNA polymerase II]]. It plays a role in the formation of the [[Pre initiation complex of RNA polymerase|Pre-Initiation complex (PIC)]] prior to the initiation of gene [[Transcription|transcription]]. Allthough eukaryotic [[Transcription initiation|transcription initiation]] is more complex than in bacteria the function of the corresponding transcription factors can be compared. Parts of TFIIF share structural homology with [[Sigma factor|sigma factor in]] bacteria. <ref name="null">Alberts, Johnson, Lewis, Raff, Roberts, Walter. (2008) Molecular Biology of The Cell. 5th Edition. p340-1</ref>  
TFIIF is a [[Eukaryotic|eukaryotic]] [[Transcription factor|transcription factor]] often found associated with [[RNA polymerase II|RNA polymerase II]]. It plays a role in the formation of the [[Pre initiation complex of RNA polymerase|Pre-Initiation complex (PIC)]] prior to the initiation of gene [[Transcription|transcription]]. Allthough eukaryotic [[Transcription initiation|transcription initiation]] is more complex than in bacteria the function of the corresponding transcription factors can be compared. Parts of TFIIF share structural homology with [[Sigma factor|sigma factor in]] bacteria&nbsp;<ref name="null">Alberts, Johnson, Lewis, Raff, Roberts, Walter. (2008) Molecular Biology of The Cell. 5th Edition. p340-1</ref>.


*The human version of TFIIF has two subunits.<ref name="1.">Whitehall S. (2011) CMB2002 lecture slides</ref>  
*The human version of TFIIF has two subunits&nbsp;<ref name="1.">Whitehall S. (2011) CMB2002 lecture slides</ref>.
*A&nbsp;major role of TFIIF in the PIC&nbsp;is&nbsp;the destabilisation of&nbsp;non-specific&nbsp;interactions between [[DNA|DNA]] and the RNA polymerase II.&nbsp; <ref name="null">Chen ZA, Jawhari A, Fischer L, Buchen C, Tahir S, Kamenski T, Rasmussen M, Lariviere L, Bukowski-Wills JC, Nilges M, Cramer P, Rappsilber J. (2010) 'Architecture of the RNA polymerase II-TFIIF complex revealed by cross-linking and mass spectrometry'. The EMBO Journal. 2010 Feb 17;29(4):717-26</ref>  
*A&nbsp;major role of TFIIF in the PIC&nbsp;is&nbsp;the destabilisation of&nbsp;non-specific&nbsp;interactions between [[DNA|DNA]] and the RNA polymerase II&nbsp;<ref name="null">Chen ZA, Jawhari A, Fischer L, Buchen C, Tahir S, Kamenski T, Rasmussen M, Lariviere L, Bukowski-Wills JC, Nilges M, Cramer P, Rappsilber J. (2010) 'Architecture of the RNA polymerase II-TFIIF complex revealed by cross-linking and mass spectrometry'. The EMBO Journal. 2010 Feb 17;29(4):717-26</ref>.
*It also&nbsp;interacts with [[TFIIB|TFIIB]] and aids&nbsp;the recruitment of [[Tfiie|TFIIE]] and [[TFIIH|TFIIH]]. Interactions between [[RNA polymerase II|RNA polymerase II and]] the [[Tata binding protein (TBP)|TATA binding protein (TBP)]] domain of [[TFIID|TFIID]] are stabilised by TFIIF. <ref>Alberts, Johnson, Lewis, Raff, Roberts, Walter. (2008) Molecular Biology of The Cell. 5th Edition. p340-1</ref>  
*It also&nbsp;interacts with [[TFIIB|TFIIB]] and aids&nbsp;the recruitment of [[Tfiie|TFIIE]] and [[TFIIH|TFIIH]]. Interactions between [[RNA polymerase II|RNA polymerase II and]] the [[Tata binding protein (TBP)|TATA binding protein (TBP)]] domain of [[TFIID|TFIID]] are stabilised by TFIIF&nbsp;<ref>Alberts, Johnson, Lewis, Raff, Roberts, Walter. (2008) Molecular Biology of The Cell. 5th Edition. p340-1</ref>.
*Experiments involving the in vitro assembly of the PIC have revealed lots about the functions of eukaryotic transcription factors. Structure comparisons between [[Mediator|mediator]]-[[RNA polymerase II|RNA polymerase II]] complexes with and without the presence of TFIIF have shown that TFIIF plays a part in maintaining the directionality of RNA polymerase II during the pre-initiation stage of transcription.<ref>Bernecky C, Grob P, Ebmeier CC, Nogales E, Taatjes DJ. (2011) 'Molecular architecture of the human Mediator-RNA polymerase II-TFIIF assembly' PLoS Biol. 2011 Mar;9(3):e1000603</ref>  
*Experiments involving the in vitro assembly of the PIC have revealed lots about the functions of eukaryotic transcription factors. Structure comparisons between [[Mediator|mediator]]-[[RNA polymerase II|RNA polymerase II]] complexes with and without the presence of TFIIF have shown that TFIIF plays a part in maintaining the directionality of RNA polymerase II during the pre-initiation stage of transcription&nbsp;<ref>Bernecky C, Grob P, Ebmeier CC, Nogales E, Taatjes DJ. (2011) 'Molecular architecture of the human Mediator-RNA polymerase II-TFIIF assembly' PLoS Biol. 2011 Mar;9(3):e1000603</ref>.
*During transcriptional [[ELONGATION|elongation]] TFIIF moves down the DNA template with RNA polymerase II.
*During transcriptional [[ELONGATION|elongation]] TFIIF moves down the DNA template with RNA polymerase II.


=== References  ===
=== References  ===


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Latest revision as of 07:19, 29 November 2011

TFIIF is a eukaryotic transcription factor often found associated with RNA polymerase II. It plays a role in the formation of the Pre-Initiation complex (PIC) prior to the initiation of gene transcription. Allthough eukaryotic transcription initiation is more complex than in bacteria the function of the corresponding transcription factors can be compared. Parts of TFIIF share structural homology with sigma factor in bacteria [1].

  • The human version of TFIIF has two subunits [2].
  • A major role of TFIIF in the PIC is the destabilisation of non-specific interactions between DNA and the RNA polymerase II [1].
  • It also interacts with TFIIB and aids the recruitment of TFIIE and TFIIH. Interactions between RNA polymerase II and the TATA binding protein (TBP) domain of TFIID are stabilised by TFIIF [3].
  • Experiments involving the in vitro assembly of the PIC have revealed lots about the functions of eukaryotic transcription factors. Structure comparisons between mediator-RNA polymerase II complexes with and without the presence of TFIIF have shown that TFIIF plays a part in maintaining the directionality of RNA polymerase II during the pre-initiation stage of transcription [4].
  • During transcriptional elongation TFIIF moves down the DNA template with RNA polymerase II.

References

  1. ↑ 1.0 1.1 Alberts, Johnson, Lewis, Raff, Roberts, Walter. (2008) Molecular Biology of The Cell. 5th Edition. p340-1 Cite error: Invalid <ref> tag; name "null" defined multiple times with different content
  2. ↑ Whitehall S. (2011) CMB2002 lecture slides
  3. ↑ Alberts, Johnson, Lewis, Raff, Roberts, Walter. (2008) Molecular Biology of The Cell. 5th Edition. p340-1
  4. ↑ Bernecky C, Grob P, Ebmeier CC, Nogales E, Taatjes DJ. (2011) 'Molecular architecture of the human Mediator-RNA polymerase II-TFIIF assembly' PLoS Biol. 2011 Mar;9(3):e1000603
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