Chromatography: Difference between revisions
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Chromatography is a very powerful method that can be used for the purification and separation of [[Molecule|molecules]] in the laboratory. For example, without chromatography, and the work of | Chromatography is a very powerful method that can be used for the purification and separation of [[Molecule|molecules]] in the laboratory. For example, without chromatography, and the work of [[Banting|Banting]], [[Best|Best]] and a biochemist called [[Collip|Collip]] (who perfected the purification), we would have no treatment for [[Diabetes|type I diabetes]], that is, [[Insulin|insulin]] would not have been discovered. Without chromatography we wouldn’t be able to sequence [[DNA|DNA]], perform [[PCR|PCR]], and many drugs and biological mechanisms would not have been discovered. | ||
Examples of common chromatography methods include: [[Ion exchange chromatography|ion exchange chromatography]], [[Size exclusion chromatography|size exclusion chromatography]], [[Affinity chromatography|affinity binding chromatography]] and [[ | Examples of common chromatography methods include: [[Ion exchange chromatography|ion exchange chromatography]], [[Size exclusion chromatography|size exclusion chromatography]], [[Affinity chromatography|affinity binding chromatography]] and [[Hydrophobic interaction chromatography|hydrophobic interaction chromatography.]] | ||
Types of chromatography include: [[Two-dimensional chromatography|two-dimensional chromatography]], [[Thin layer chromatography|thin layer chromatography]] ([[Thin layer chromatography|TLC]]) and [[Paper chromatography|paper chromatography]]. | |||
Revision as of 21:05, 8 December 2011
Chromatography is a very powerful method that can be used for the purification and separation of molecules in the laboratory. For example, without chromatography, and the work of Banting, Best and a biochemist called Collip (who perfected the purification), we would have no treatment for type I diabetes, that is, insulin would not have been discovered. Without chromatography we wouldn’t be able to sequence DNA, perform PCR, and many drugs and biological mechanisms would not have been discovered.
Examples of common chromatography methods include: ion exchange chromatography, size exclusion chromatography, affinity binding chromatography and hydrophobic interaction chromatography.
Types of chromatography include: two-dimensional chromatography, thin layer chromatography (TLC) and paper chromatography.