Sticky ends

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Sticky ends are fragments of DNA where one strand, after being cleaved by restriction enzymes, is left over hanging another strand. These overhangs are termed 'sticky' because they can easily bind to free nucleotides [1].

Some examples of Restriction endonucleases that cut to create 'sticky ends' are:

BamHI, BaI228I and EcoRI [2]

References

  1. ↑ http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html
  2. ↑ http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_E-F#Whole_list_navigation


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