Sticky ends

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Sticky ends are fragments of DNA where one strand, after being cleaved by restriction enzymes, is left over hanging another strand. It is cleaved assymetrically and can leave a 5' or a 3' overhang. These overhangs are termed 'sticky' because they can easily bind to free nucleotides [1]. Restriction enzymes can also symmetrically cleave DNA strands to form blunt ends.

Some examples of Restriction endonucleases that cut to create 'sticky ends' are:

[2]

References

  1. ↑ http://www.genscript.com/product_003/molecular_biology_glossary/id/12153/category/glossary/Sticky_ends.html
  2. ↑ http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_E-F#Whole_list_navigation


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