Electroporation
Electroporation is a method used to insert a recombinant plasmid into a bacterial cell using high voltage electrical pulses[1].
In molecular biology, electroporation is commonly used for cell transfection or transformation of non-viral DNA transfer of bacteria, yeast, and plant plastids. Electroporation is also highly effective for the introduction of foreign genes into tissue culture cells, especially mammalian cells[2].
Example of electroporation:
E. coli plasmid insertion with CaCl2 on ice with heat shock.
References
- ↑ http://www.lifetechnologies.com/my/en/home/life-science/cell-culture/transfection/transfection-methods/electroporation.html
- ↑ Jaquith K. What Is Electroporation? - Universal Medical Blog [Internet]. Universal Medical Inc. Blog2017 [cited 2017 Nov 16];Available from: https://blog.universalmedicalinc.com/what-is-electroportation/