Mass spectrometry

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Mass Spectrometry in the process in where proteins can be identified. Whether it be a single protein, or a collections of proteins, obatined after procedures such as gel electrophoresis or by column chromatography.The proteins in the sample are firstly broken down into smaller peptides, where they are ionized creating a postive charge. These ionized peptides are accelerated via an electric field towards a decetor. The mass to charge ratio of these ions affect the time it takes for them to reach the dector, large peptides moving slower and higher charged molecules move faster.[1] The masses of the peptides obtained by the detector can then be measured and compared to other well known peptides in a database, determing the genomic seqeunce and ultimately the protein.   

References

  1. Alberts et al (2008) Molecular Biology Of The Cell, 5th Edition

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