Ethidium Bromide: Difference between revisions

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Ethidium Bromide, commonly used during [[gel electrophoresis|gel electrophoresis]] in molecular biology labs, is a fluorescent tag which intercalates with [[DsDNA|double-stranded DNA]] and [[RNA|RNA]] (due to it's tendancy to fold back on itself and create localised sections of [[Watson-Crick|Watson-Crick]] interaction, and emits an orange glow when under [[UV|UV]] light.
Ethidium Bromide is commonly used during [[Gel electrophoresis|gel electrophoresis]] in molecular biology labs. The Ethidium Bromide intercalates itself between&nbsp;[[Base pair|base pairs]] allowing the [[DNA|DNA]] banding pattern to be visualised when illuminated with a [[Ultra Violet|UV]] light source.&nbsp;As might be expected, the addition of ethidium bromide will affect the mobility and rigidity of the DNA molecule through the [[Agarose gel|agarose gel]]&nbsp;<ref>http://www.ncbi.nlm.nih.gov/pubmed/8957173</ref>.<br>
 
=== References  ===
 
<references />

Latest revision as of 23:04, 1 December 2015

Ethidium Bromide is commonly used during gel electrophoresis in molecular biology labs. The Ethidium Bromide intercalates itself between base pairs allowing the DNA banding pattern to be visualised when illuminated with a UV light source. As might be expected, the addition of ethidium bromide will affect the mobility and rigidity of the DNA molecule through the agarose gel [1].

References

  1. ↑ http://www.ncbi.nlm.nih.gov/pubmed/8957173
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