DNA ligase: Difference between revisions
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DNA ligase is an enzyme that anneals [[DNA|DNA]] strands together. It forms a phosphodiester bond between the complementary bases on two separate DNA strands. [[ATP|ATP]] is hydrolized in order to release a pyrophosphate, and the [[AMP|AMP]] that is formed attaches to the 5' end. The [[pyrophosphate|pyrophosphate]] bond activates the 5' end and a [[phosphodiester bond |phosphodiester bond]] forms between the [[5' phosphate|5' phosphate]] and the [[3' hydroxyl|3' hydroxyl]]. DNA ligase is a crucial element in recombinant technology <ref>Alberts, Bruce. Molecular Biology of the Cell. 5th ed. New York: Garland Science, 2008, p 273</ref>. | |||
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Revision as of 18:30, 21 October 2012
DNA ligase is an enzyme that anneals DNA strands together. It forms a phosphodiester bond between the complementary bases on two separate DNA strands. ATP is hydrolized in order to release a pyrophosphate, and the AMP that is formed attaches to the 5' end. The pyrophosphate bond activates the 5' end and a phosphodiester bond forms between the 5' phosphate and the 3' hydroxyl. DNA ligase is a crucial element in recombinant technology [1].
References
- ↑ Alberts, Bruce. Molecular Biology of the Cell. 5th ed. New York: Garland Science, 2008, p 273