Electrophoresis: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. | [[SDS polyacrylamide-gel electrophoresis|Electrophoresis]] is used as a method of separating [[DNA|DNA]] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.<br> | ||
Revision as of 15:17, 1 December 2011
Electrophoresis is used as a method of separating DNA according to size, using a porous gel as a filter. By applying an electric current, the molecules of DNA are made to move through the gel towards the positive electrode (anode), with the smaller or more compact strands travelling the fastest. This produces distinct bands of DNA, which can be identified by comparing with a known DNA sample 'ladder' run simultaeously. Any molecule with a net charge, such as proteins, can also be seperated using this technique.