Electrophoresis: Difference between revisions
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Electrophoresis is used as a method of separating [http://bms.ncl.ac.uk/wiki/extensions/FCKeditor/fckeditor/editor/DNA DNA] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest. This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. | Electrophoresis is used as a method of separating [http://bms.ncl.ac.uk/wiki/extensions/FCKeditor/fckeditor/editor/DNA DNA] according to size, using a porous gel as a filter. By applying an electric current, the [[Molecule|molecules]] of [[DNA|DNA]] are made to move through the gel towards the positive electrode ([[Anode|anode]]), with the smaller or more compact strands travelling the fastest.<ref name="Gel Electrophoresis Virtual Lab">http://learn.genetics.utah.edu/content/labs/gel/</ref> This produces distinct bands of [[DNA|DNA]], which can be identified by comparing with a known [[DNA|DNA]] sample 'ladder' run simultaeously. | ||
Revision as of 15:35, 23 November 2010
Electrophoresis is used as a method of separating DNA according to size, using a porous gel as a filter. By applying an electric current, the molecules of DNA are made to move through the gel towards the positive electrode (anode), with the smaller or more compact strands travelling the fastest.[1] This produces distinct bands of DNA, which can be identified by comparing with a known DNA sample 'ladder' run simultaeously.