Β-galactosidase: Difference between revisions
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'''Beta-galactosidase''' (B-galactosidase), also known as '''lactase''', is encoded by the '''lacZ gene''' that is commonly found in the plasmid of the '''bacterium '''''[[ | '''Beta-galactosidase''' (B-galactosidase), also known as '''lactase''', is encoded by the '''lacZ gene''' that is commonly found in the plasmid of the '''bacterium '''''[[Escherichia coli|'''E. coli''']]''. This gene resides within the [[Lac operon|'''lac operon''']], regulated by the '''[[Lac_repressor|lac repressor]]''' and '''CAP'''. B-galactosidase is an [[Enzyme|'''enzyme''']] that '''[[Catalyse|catalyses]] '''the [[Hydrolysis|'''hydrolysis''']] of a [[Disaccharide|disaccharide]], [[Lactose|'''lactose''']], into the two [[Monosaccharide|monosaccharides]], [[Glucose|'''glucose''']] and [[Galactose|'''galactose''']]<ref>Alberts. A, Johnson. A, Lewis. J, Morgan. D, Raff. M, Roberts. K, et al. Molecular Biology of the Cell. 6th ed. New York: Garland Science. 2015.</ref>. | ||
<span style="font-size: 13.28px;">Plays an essential part in </span>[[Blue/white Selection|blue/white selection technique]] | <span style="font-size: 13.28px;">Plays an essential part in </span>[[Blue/white Selection|blue/white selection technique]] | ||
=== Reference: === | === Reference: === | ||
<references /> | <references /> | ||
Revision as of 21:00, 20 October 2018
Beta-galactosidase (B-galactosidase), also known as lactase, is encoded by the lacZ gene that is commonly found in the plasmid of the bacterium E. coli. This gene resides within the lac operon, regulated by the lac repressor and CAP. B-galactosidase is an enzyme that catalyses the hydrolysis of a disaccharide, lactose, into the two monosaccharides, glucose and galactose[1].
Plays an essential part in blue/white selection technique
Reference:
- ↑ Alberts. A, Johnson. A, Lewis. J, Morgan. D, Raff. M, Roberts. K, et al. Molecular Biology of the Cell. 6th ed. New York: Garland Science. 2015.