Restriction digests

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Restriction digests also known as restriction enzymes/endonucleases. They are enzymes found in bacteria. They cut specific sequences of DNA: 4 to 8 base pairs approximately at sites called recognition sites. The endonucleases cleave the sugar-phosphate backbone of DNA. Restriction digests commonly used are EcoRI, BamHI and HindIII.

When the enzyme cleaves the DNA- they generally produce 3 different types of cleavage:

After restriction digest, DNA can then be analysed using agarose gel electrophoresis. This is very useful in recombinant DNA therapy [1].

References

  1. http://amrita.vlab.co.in/?sub=3&brch=77&sim=694&cnt=1

 

 

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